p53 is an important tumor suppressor gene. The p53 pathway is activated in response to cellular stress stimulation. However, in more than 50% of breast cancers, p53 is mutated or inactivated, which permits cancer growth. Although a large number of co-regulators of p53 have been identified, the integrated molecular network of p53 and the key co-factors that could transactivate p53 remain unclear. Myeloid Zinc Finger 1 (MZF1), a member of the SCAN-ZFP (SCAN-Zinc finger protein) family, has been involved in the occurrence and development of various types of malignant tumors, including breast cancer. However, the exact mechanism of action remains unclear. Our study aims to investigate the cross-regulatory loop between MZF1 and p53 in breast cancer cells.