Mutant KRAS is a common driver in lung cancer. Nevertheless, the occurrence of human lung cancer and its subsequent development after activation of oncogenic KRAS in epithelial cells remain poorly understood. Organoids are embedded in three-dimensional Matrigel in which tissue-derived human adult stem cells can efficiently grow for a long time, maintaining genetic and phenotypic stability. But until now, the use of organoids to simulate human non-small cell lung cancer has not been reported.