Colorectal cancer (CRC) is a common cancer with high morbidity and mortality. Post-translational modification (PTM) of protein plays an important role in the pathogenesis of CRC. Lysine crotonylation (Kcr) is an important type of PTM and has been proved evolutionarily conserved in eukaryotic cells from a wide range of species. However, the role of protein Kcr in the progression of CRC is unclear.