The loss of a pregnancy, including ectopic pregnancy (EP) and early pregnancy loss (EPL), significantly impacts women’s quality of life. Unfortunately, definitive causes can be identified in less than half of EP and EPL cases, presenting a substantial challenge for clinical treatment. Previous studies have revealed a significant relationship between the history of EPL and the increased risk of EP.1 Nevertheless, the interplay between EPL and EP remains unclear, highlighting the need to discover novel biomarkers to guide personalized treatment and clinical management. In response, we aim to investigate the underlying genetic interactions in EPL and EP. Using various bioinformatics analyses, we examined the genetic interactions of differentially expressed genes (DEGs) through RNA sequencing according to the workflow (Fig. S1).