Female breast cancer (BCa) has overtaken lung cancer as the most diagnosed cancer worldwide.1 The overall survival for BCa patients without metastasis for five years is over 80%.2 However, relapse and metastasis remain the primary challenge for BCa. In various carcinomas, Holliday junction recognition protein (HJURP) has shown up-regulated patterns and belongs to the CENP-A pre-nucleosomal complex.3, 4, 5 HJURP's expression pattern, roles, and prognosis remain largely unknown in BCa. Nevertheless, we investigated HJURP's expression in BCa and explored its possible role in cancer development. We found that HJURP was significantly higher in BCa tissues and associated with pathological characteristics, and down-regulation of HJURP in BCa cells could suppress the proliferation and migration of MDA-MB-231 cells through the PI3K-AKT pathway. A significant role for HJURP in BCa progression has been identified, and it could provide a valuable biomarker for the diagnosis and prognosis of BCa. Also, considering HJURP as the novel diagnosis and treatment target will provide new strategies for early diagnosis, precise treatment, and effective prevention of BCa.