Pancreatic cancer (PC) is an aggressive malignant tumor with low rate of surgical resection and poor prognosis. Transforming growth factor-β (TGF-b) is a cytokine that has both protumor and antitumor activities, depending on tumor microenvironment. The interaction between TGF-b signaling and the tumor microenvironment in PC is complex. Here, we reviewed the role of TGF-b in the tumor microenvironment of PC, highlighting producers of TGF-b and TGF-b responders in the tumor microenvironment of PC.