Lipoprotein-associated phospholipase A₂ (Lp-PLA₂), a member of the phospholipase A₂ superfamily, isanenzymethathydrolysesphospholipids, isfoundinbloodcirculationasasign of inflammation, and takes a role in atherogenesis. There is an epidemiologic relation between increased Lp-PLA₂ levels and coronary heart disease. Lp-PLA₂ is an enzyme that is produced by macrophagesandtakesaroleasanindependentpredictorofacoronaryevent. Ageneticvariant of Val279Phe on the Lp-PLA₂ gene has been reported with various results in Japan, China, Korea, and Caucasian populations. This study aims to analyse the influence of the Val279Phe genetic variant on acute myocardial infarction (AMI) at Saiful Anwar Hospital, Indonesia. This study was conducted on 151 patients (111 AMI patients and 40 non-AMI patients). The genetic variant of Val279Phe was identified through a genotyping method. There were nosignificant differences in age, total cholesterol level, LDL-C (low-density lipoprotein cholesterol) level, and family history data between AMI and non-AMI patients. However, AMI patients had low HDL-C (high-density lipoprotein cholesterol), triglyceride levels, dyslipidaemia, and hypertension risk factors compared to non-AMI patients. The frequency of the GG genotype (279Val) was dominant in both AMI and non-AMI groups. Further analysis suggested that the GG genotype has a 2.9 times greater risk of AMI compared to the GT/TT genotype (279Phe). This study concluded that the Val279Phe genetic variant undoubtedly influenced AMI risk, which is a warrant for further development of early detection and improving strategy to prevent AMI in patients.