Androgenic alopecia (AGA) is the most common type of clinical alopecia. Androgen receptor (AR) is the most logical candidate gene for regulating the occurrence of AGA. Dermal papilla cells (DPCs) are a special kind of mesenchymal cells, located in the hair bulb of hair follicles. DPCs play a role in maintaining and inducing the periodic cycling of hair follicles, and are considered as a key cell target of androgen in hair follicles. Tcf4 is a positive regulator of the maintenance of DPC biological features. Previously, we reported that Twist1 can enhance the inductive effect of Tcf4. As a transcription factor, AR can bind with Tcf4 to regulate the proliferation in prostate growth and tumorigenesis. However, whether and how AR interacts with Tcf4 in DPCs remains unknown.