Here, we developed a comprehensive web-searchable database, designated as Human RNA Modifications Disease Database (HRMDD, http://bio-bigdata. hrbmu. edu.cn/HRMDD/home.jsp). RNA modification (RM) is an important mechanism of epigenetic regulation. With the evolution of both experimental technologies and computational methods, major progress has been made in identifying the genomic locations and distributions of various RM types throughout the transcriptome. Additional breakthroughs came from the identification and characterization of RM regulators. RMs are generally regulated by three different types of regulators, which are deposited, removed, and recognized by proteins known as "writers", "erasers" and "readers", respectively. Increasing evidence suggests that dysregulation of RMs and their regulators is implicated in various cancers, as well as other diseases. Despite these advances in understanding RMs and their regulators, their biological functions and mechanisms in human diseases remain largely unknown.