Neuropathic pain (NP) is a chronic condition with complex molecular mechanisms. Jakyakgamcho-tang (JGT) is derived from Paeoniae Radix and Glycyrrhizae Radix et Rhizoma, and shows a significant therapeutic potential for pain management,1 but its underlying mechanisms remain unclear. This study aimed to elucidate the mechanism of action of JGT in NP by systematically investigating the key NP-associated transcription factors (TFs) and signaling pathways regulated by JGT and its compounds. Additionally, we analyzed the transcriptome of JGT-treated PC12 cells and performed in vitro experiments to confirm the regulation of target genes.